- #Flowjo 10 make histograms match how to
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Yep, that's right, good ole GFP finally getting the props it deserves. The prize will be shared among Osamu Shimomura, Martin Chalfie, and Roger Tsien for the discovery and development of green fluorescent protein, GFP. In light of the yesterday's announcement of the 2008 Nobel Prize in Chemistry, I'd like to take a minute to demonstrate the importance of this work. Or, just drop by on either day to take a look at the setup. We have a few scheduled demos of the laser with users of the facility, so if you'd like to see the laser in action, give us a call to find out what time we'll be running samples on the 2nd and 3rd. This will effectively increase the resolution of dimly stained populations, especially when identifying cells which are double positive for FITC and PE. Other benefits of interest will be the stronger excitation of PE and PE-Tandems as well as the lack of spillover of blue excited fluorochromes like FITC and PerCPCy5.5 into PE channels. The major benefit of this laser will be the ability to excite fluorochromes not normally excited at 488nm or 633nm, such as mCherry, RFP variants, and more.
#Flowjo 10 make histograms match install
Beckman Coulter will be in to install the laser as well as a detector block so we can assess the utility of such a laser for our sorting applications. We will be having a demo of a 200mW 561nm solid state laser on our MoFlo December 2nd and 3rd, 2008. Classes start 12/10/08, so check the online scheduler for more dates and to sign up.
![flowjo 10 make histograms match flowjo 10 make histograms match](https://present5.com/presentation/6b59495f6cd398f7ee6ce32f6ae8f385/image-67.jpg)
We've embedded the video here for you to watch.
#Flowjo 10 make histograms match how to
So, we also made a little video demonstration on how to sign-up for training. Although the Online Scheduler offers convenience and flexibility, the sign-up process has a few steps to it that may make it somewhat challenging the 1st time you attempt. We're also putting the course schedule on the facility's Online Instrument Scheduler, so now, you'll know exactly when the next class will be offered, as well as have an opportunity to sign up whenever is convenient for you. Once the initial rush is over, we will over the course every other week as new users are being trained. To make this process easier, we will be offering the course more frequently especially in the next coming months. We will once again be making this a mandatory requirement for new flow cytometer trainees, but anyone who did not attend the original flow basics, will want to attend one of these training sessions. We've trimmed a bunch of the slides while still focussing on the most important aspects of basic flow cytometry theory and instrument usage. You've gotta be down to, what, like 5MB or something? Not only is Flow Basics back, but it's leaner and meaner than ever, well, not really meaner, but definitely leaner. But, Wow Flow Basics, have you been working out? I mean, you're about half the size as the last time I saw you.
![flowjo 10 make histograms match flowjo 10 make histograms match](https://expert.cheekyscientist.com/wp-content/uploads/2014/09/5a.png)
That's right folks, by popular demand, we're reintroducing a legend, Flow Basics. I've already got a name for it.Medussa! If you have a high enough laser source, you could split that beam a bunch of times, and using fiber optic cables, bring the light to any number of instruments using a "plug-n-play" connection scheme.
![flowjo 10 make histograms match flowjo 10 make histograms match](https://docs.flowjo.com/wp-content/uploads/sites/6/2013/03/com.treestar.flowjo.main_.Main-1.png)
If successfully employed, these types of technologies may trickle their way down to us lowly folks in the flow cytometry world, and I may actually be able to realize my goal of a centralized laser depot with a bunch of fiber-optic cables extending out of it. These so-called slab lasers are attempting to tackle a long-standing problem with lasers, what to do with waste heat.
#Flowjo 10 make histograms match series
So, what's so special about the laser? It seems this laser system is using a series of thin ceramic slabs, bathed in rapidly circulating coolant to achieve a high power output while maintaining a non-destructive temperature load. HELLADS, or High Energy Liquid Laser Area Defense System, a laser system developed by Textron, is getting some big government bucks to design and test a 150kW Laser Weapon System that could be carried on a Fighter Jet and be used to shoot down missiles. Darpa (The advanced research division of the Department of Defense) has been investigating weapons-grade lasers for some time, but with the use of some newer technologies, there may actually be a viable option. Lasers are used all over the place, most-notably for me, Flow Cytometry and Microscopy, but now it seems like the vision of Star Wars may be closer than ever. Another benefit is that light travels, well, at the speed of light, much faster than a speeding bullet or a falling bomb. No need to carry along a bunch of ammunition, all you need is your trusty multi-KiloWatt laser. It just seems like such a practical way to shoot things. I'm not one of those freaky, dress-up like Chewbacca fans, and talk like yoda, I do not, but what I really like about Star Wars are the lasers.